- Hapten model induction
- Sensitization and repeated challenge with oxazolone / topically on back and ear
- Immunocompetent rodent sp. (Balb/c strain)
- Clinical endpoints / scoring (equivalent to SCORAD):
- Back skin dryness/scaling
- Back skin redness/erythema
- Ear and back skin thickness/edema
- Back skin excoriations
- Itching (observed behaviour)
- Ethical endpoints: bodyweight / feeding & drinking behavior / general behavior / maximal scoring threshold
- Corticoid (betamethasone, clobetasol) or immunosuppressor as positive control
- Blood samplings for circulating (cytokines)
- Skin biomarkers analysis
- Ex vivo imaging (histology HES / IHC / electron microscopy (SEM))
- Statistical analysis of efficacy data: clinical scoring and histomorphometrics
Induction with other chemicals for haptens induction (calcipotriol / DNCB) is also available
Please inquire for more details on Oxazolone induced Atopic Dermatitis Model